Sisir Dutta

Sisir K. Dutta
Professor Emeritus
Graduate Faculty

Ph.D. 1960. Kansas State University

Department of Biology
415 College Street, NW
Washington, D. C. 20059
Telephone: (202) 806-6942
Fax (202) 806-5832
Office Room 335
Lab Room 349 and 449
sdutta@howard.edu

S. Dutta's Lab

RESEARCH:

Currently Dr. Dutta and his group are working on gene expression profiling and pathway construction using entire human genome oligonucleotide microarrays (affymetrix HG-133 Plus 2.0). They have discovered for the first time some genomic biomarkers relevant to human diseases induced by PCBs.

Our laboratory has been involved in isolation of specific genes from organisms and studied their expressions under different environmental stresses. As early as 25 years ago (1972) our laboratory was able to isolate highly purified ribosomal RNA and transfer RNA genes from the filamentous fungus Neurospora crassa for the first time. These genes were fully characterized as to their sizes, numbers of repeat units and their location in the genomes using DNA: RNA and DNA:DNA re-association kinetics technology. Later on we extended these studies in studying neuron specific enolase (NSE) and non-neuron enolase specific (NNE) genes from mammalian neuron cells, various oncogenes, p53 genes and a few other housekeeping genes. The phylogenetic implications of these genes have been studied in great length and have been published in three books entitled "DNA SYSTEMATICS" as listed later.

Our molecular genetics laboratory has been working on several aspects of genotoxic effects of different environmental stresses such as non-thermal electromagnetic radiations, mutational and carcinogenic effects of hazardous chemical pollutants such as explosive TNT and fire retardant PCB. Using state of the art PCR Technology, cloning, and DNA sequencing we are now able to isolate and identify microorganisms which exist underneath the ocean, rivers and undergrounds. These studies (particularly using 16S rDNA) have enabled us to identify some anaerobic microbes, such as Clostridium, which degrades PCBs, which have not been identified before, even though scientists have tried for last 10-12 years. We are also using a white rot fungus Phanerochaete chrysosporium in biotransformation studies and in the search for 2,4-DNT degrading gene(s), which is one of the toxic metabolites of TNT. We have demonstrated that Rhizobium sp. can also bioremediate contaminated soil with petroleum products like toluene. Several undergraduate, graduate and post-doctoral students are currently working on various funded projects in our well equipped laboratories.

We have published five books and more than 165 articles in various journals. Dr. Dutta and his several students have received national recognitions and monetary awards from various sources.

TEACHING:

Molecular Genetics (Both undergraduate and graduate levels). The course deals with molecular genome analysis, gene isolation using DNA:DNA and DNA:RNA re-association kinetics, molecular evolution, microbial ecology using 16S rDNA-ARDRA (Amplified Ribosomal DNA Restriction Analysis) techniques, genes for bioremediation, and DNA-Diagnostic tools (RFLP, RT-PCR, DNA-Chips and Microarray). Other courses taught are general Biology and general Microbiology.

AWARDS:

Current recipient of Agilent Instrument Award for LC-EIS-TOF-MS (PI. Dr. F. Ayorinde & Co-PI Dr. S. K. Dutta). First Prize for outstanding faculty research by ASB (American Society of South-Eastern Biologists) 2004; Faculty Author Awards 1996-2005; Nominated for Life time Mentoring Award by AAAs 2002. Outstanding Faculty Award by Howard University Students (1994); Distinguished Teacher/Scholar Award (1994), Howard University Cluster; HALL OF FAME for contribution in Molecular Genetics; NIH recognition as Outstanding Minority Student Mentor in Research (1998); Outstanding Research Award, College of Liberal Arts, 1983; Great Teacher Award by MERCK Pharmaceutical Company; WHO’S WHO of USA and the World; WHO’S WHO in Frontier Sciences; Recipient of approx. 5 million dollars research grants as PI and several million more dollars Institutional Training grants.

STUDENT AWARDS AND MENTORING:

First Prize winner in the Graduate Research Symposium 2005 (Supriyo De) for PhD work, Howard University, Washington DC; US/India research award (Supriyo De) 2005-2006. Howard University, Washington DC; First Prize winner in the Graduate Research Symposium 2002 (Supriyo De) for MS work. Howard University, Washington DC. Several other graduate students: Michelle Jackson, Christine Barrow, LaShaun Drew, Gail Hollowell, Gregory Davenport, Christie Maggee and several other graduate students received several awards from Graduate school and at national and international levels.

Mentors for following numbers of students; Undergraduate Research Students including Minority Alliance Program- 45; Numbers of graduate (M.S. & Ph.D.) research students-90; Numbers of post-doctoral research students-25; Judges in local elementary, middle, and high school science fairs-4; Total numbers of research students trained in the areas of molecular genetics during 38 years tenure at Howard University- 162. Most of his prior students are currently holding prized positions in U.S Government Offices, in Universities and in Industries.

PUBLICATIONS:

Publications Beginning January 2006 (selected out of 165+ peer reviewed journal papers)

De S, Perkins M, Dutta SK., Nitrate Reductase Gene Involvement in Hexachlorobiphenyl Dechlorination by Phanerochaete chrysosporium. J. Hazardous Materials 2006, (In press; doi:10.1016/j.jhazmat.2005.11.073).

Chen Y-Q, Adam A, Toure O, and Dutta SK, Molecular evidence of genetic modification of Sinorhizobium meliloti : enhanced PCB bioremediation, J. Indus. Microbiol. Biotechnol., 32: 77-85, 2005.

Perkins MW, De S, Frederick L, Dutta S K ,Ligninolytic Mineralization of Trinitrotoluene by Several White Rot Basidiomycetes. J. of Bioremediation, 9: 77-85, 2005.

De S, Pramanik SK, Williams AL, Dutta S K, Toxicity of Polychlorobiphenyls and its Bioremediation. Int. J. of Human Genetics, 4: 281-290, 2004.

Banerjee H., Verma M., Dutta S.K.; 2-Am,4,6-DNT Causes Genotoxicity of P53 Gene in NG108 Neuroblastoma Cell Lines, Int. J. Hum. Genet., 4: 227-229, 2004.

Dutta, S.K., Adam A., Toure O., Williams A.L. and Chen Y.Q. Indigenous mixed soil bacteria in presence of compatible plants are more efficient in PCB degradation. F. Environ. Bull, 12: 314-319, 2003.

Toure, O., Chen Y.Q., and Dutta S.K. Sinorhizobium meliloti electrotransporant containing ortho-dechlorination gene shows enhanced PCB-dechlorination. F. Environ. Bull, 12:, 320-322, 2003.

Dutta, S.K., Hollowell, G.P., Fawzy, M.H., and Kuykendall, L.D. Genetically modified Sinorhizobium meliloti significantly enhances 2,4-DNT contaminated soil bioremediation. Soil Biol. and Biochem. 35: 667-675, 2003.

Drew L and Dutta S.K. DNA diagnostic tools for β-hemoglobinopathies. Proceeding 10 -12, 2001.

Dutta, S.K. and Toure, O. Accelerated Depletion of 2’, 3, 4 PCB congener using combinations of alfalfa plant and bacterial species. Proc. Hazardous Waste Research. pp. 1-4, 2000.

Hou Li-Hua and Dutta S.K.. Phylogenetic characterization of several para-and meta-PCB dechlorinating Clostridium species: 16S rDNA sequence analyses. Letters in Applied Microbiology, 30: 238 -243, 2000.

Jackson, M.M., Hou, L.-H., Banerjee, H.N., Sridhar, R., and Dutta, S.K. Disappearance of 2,4-dinitrotoluene and 2-amino, 4, 6-dinitrotoluene by Phanerocheate chrysosporium under non-lignolytic conditions. Bull. Environ. Contam. Toxicol. 62:390-396, 1999.

Hollowell G.P., Kukendall L.D., Gillette W.K., Hashrem F.M., Hou L.H., Tatem H.E., and Dutta S.K. Genetic transfer and expression of plasmid RP4:TOL in Sinorhizobium meliloti, Bradyrhizobium japonicum and B. elkanii. Soil Biology and Biochemistry 31: 1811-1819, 1999.

Dutta, S.K., Jackson, M.M., Hou, L.-H., Powell, D., and Tatem, H. Non-ligninolytic TNT mineralization in contaminated soil by Phanerochaete chrysosporium. Bioremediation J. 2(2): 97-103, 1998.

Dutta, S.K., Nazar, A.S.M.I. and Verma, M.: Enolase activity in chicken embryo primary retina cells is not affected by exposure to a 60-Hz electric field. Cancer Biochemistry Biophysics 16: 45-52, 1998.

LaMontagne, M.G., Davenport, G.J., Hou, L-H., and Dutta, S.K. Identification and analysis of PCB dechlorinating anaerobic enrichments by amplification: Accuracy of community structure based on restriction analysis and partial sequencing of 16S rRNA genes. J. Appl. Microbiol. 84(6): 1156-1162, 1998.

Dutta S.K., Hou L.H. , Jackson M.M. and Tatem H.E. In situ disappearance of TNT from contaminated soils using microbes and co-metabolites. Proc. WERC/HSRC Cont. on the Environment 111-112, 1997.

Kuykendall, L.D., Gillette, W.K., Hollowell, G.P., Hou, L.-H., Tatem, H.E., and Dutta, S.K.: Transfer Plasmid RP4::TOL to Legume Microsymbionts for Soil/Rhizosphere Bioremediation. In In Situ and On-Site Bioremediation, Vol. 4 (4): Battelle Press, Columbus, OH, pp65-70, 1997.

Nazar, A.S.M.I., Paul, A. and Dutta, S.K.: Frequency-dependent alteration of enolase activity by ELF fields. Bioelectrochemistry and Bioenergetics 39: 259-262, 1996.

Verma, M. and Dutta, S.K.: Transcription initiation and termination sites of ribosomal RNA gene clusters of Neurospora crassa. The Nucleus 39(1,2): 35-42, 1996.

Dutta S.K. and Verma, M.: New directions in genetic-therapy research. Ind.J.Hum.Genet. 2: 13-18, 1996.

Verma, M and Dutta S.K.: Transcription initiation and termination sites of ribosomal RNA gene clusters of Neurospora crassa. The Nucleus 39 (1,2): 35-42, 1996.

Verma, M. and Dutta, S.K.: DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899, 1994.

Dutta, S.K., Verma, M., and Blackman, C.F.: ELF frequency-dependent alterations in enolase activity in Esherichia coli caused by exposure to electric and magnetic fields. Bioelectromagnetics 15: 377-383, 1994.

Nazar, A.S.M.I. and Dutta, S.K.: Effect of ELF magnetic fields on enolase activity. Electro and Magnetobiol. 13: 175-181, 1994.

Kuykendall, L.D., Gaur, Y.D, and Dutta, S.K.: Genetic diversity among Rhizobium strains from Cicer arietinum L. Letters in Applied Microbiol, 17: 259-263, 1993.

Banerjee, D., Schnieders, B., Bertino, J.R. and Dutta, S.K. Detection of a novel double mutation in a ?-thalassemia patient by RNA single-stranded conformation polymorphisms and direct sequencing. Blood 82: 3786-3792, 1993.

Banerjee D, Talukder G, Tiwari R, Bhattacharya DK, Pakrashi A, Sharma A, Banerjee A, De M, Winter WP, Dutta SK: Molecular diagnosis of several β-Thalassemic mutations in patients from Calcutta, West Bengal populations using DNA probes and PCR. Nucleus, 35: 106-110, 1992.

Dutta, S.K. & Verma, M.: Primary structure of the non-transcribed spacer region and flanking sequences of the ribosomal DNA of Neurospora crassa and comparison with other organisms. Biochem. Biophys. Res. Comm. 170: 187-193, 1990.

Dutta, S.K., Bhattacharyya, Nandan, Parui, R. & Verma, Mukesh: Expression of DNA sequences containing neuron specific enolase gene in Escherichia coli. Biochem. Biophy. Res. Comm.173: 231-239, 1990.

Dutta, S.K. & Verma, M. Isolation and characterization of non-neuronal enolase (NSE) from Neurospora crassa and comparison with neuron specific enolase isolated from neuroblastoma cell line NG108. Biochem. Intl. 15: 223-234, 1987.

Dutta, S.K., Mukhopadhyay, D.K. & Bhattacharya, J. RNase sensitive DNA polymerase activity in cell fractions and mutants of N.crassa. Biochem. Genet. 18: 743-753, 1980.

Dutta, S.K., Beljanski, M. & Bourgarel, P.: Endogenous RNA bound RNA- dependent DNA polymerase activity in N. crassa. Exptl. Mycol. 1: 173-180, 1976.

Chattopadhyay, S.K., Kohne, D.E. & Dutta, S.K. Ribosomal RNA genes of Neurospora: Isolation and characterization. Proc. Natl. Acad. Sci. USA 69: 3256-3259, 1972.

Garber, E.D. & Dutta, S.K. Evidence of mixed cytoplasm in heterocaryons of C.lagenarium. Science 135: 665, 1962.

BOOKS

Dutta, S.K. and Sloger, C. (Eds.). Nitrogen Fixation Associated with Rice Production. Oxford & IBH Publishing Co., New Delhi, India (1991). ISBN # 81-204-0560-9.

Dutta, S.K. and Winter, W.P. (eds.). DNA Systematics: Human and Higher Primates, Vol. III, CRC Press, Florida (1990). ISBN # 0-8493-5822-1.

Dutta, S.K. and Millis, R.M. (eds.). Biological Effects of Electropollution: Brain Tumors and Experimental Models. Information Ventures, Inc., Philadelphia, PA (1986). ISBN # 0-9617314-1-9.

Dutta, S.K. (ed.). DNA Systematics: General, Vol., CRC Press, Florida (1986). ISBN # 0-8493-5820-5.

Dutta, S.K. (ed.). DNA Systematics: Plants, Vol. II., CRC press, Florida (1986). ISBN # 0-8493-5821-3.


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		Sisir K. Dutta Professor Emeritus Graduate Faculty Ph.D. 1960. Kansas State University
			Department of Biology 415 College Street, NW Washington, D. C. 20059 Telephone: (202) 806-6942 Fax (202) 806-5832 Office Room 335 Lab Room 349 and 449 sdutta@howard.edu
				S. Dutta's Lab

	RESEARCH: Currently Dr. Dutta and his group are working on gene expression profiling and pathway construction using entire human genome oligonucleotide microarrays (affymetrix HG-133 Plus 2.0). They have discovered for the first time some genomic biomarkers relevant to human diseases induced by PCBs. Our laboratory has been involved in isolation of specific genes from organisms and studied their expressions under different environmental stresses. As early as 25 years ago (1972) our laboratory was able to isolate highly purified ribosomal RNA and transfer RNA genes from the filamentous fungus Neurospora crassa for the first time. These genes were fully characterized as to their sizes, numbers of repeat units and their location in the genomes using DNA: RNA and DNA:DNA re-association kinetics technology. Later on we extended these studies in studying neuron specific enolase (NSE) and non-neuron enolase specific (NNE) genes from mammalian neuron cells, various oncogenes, p53 genes and a few other housekeeping genes. The phylogenetic implications of these genes have been studied in great length and have been published in three books entitled "DNA SYSTEMATICS" as listed later. Our molecular genetics laboratory has been working on several aspects of genotoxic effects of different environmental stresses such as non-thermal electromagnetic radiations, mutational and carcinogenic effects of hazardous chemical pollutants such as explosive TNT and fire retardant PCB. Using state of the art PCR Technology, cloning, and DNA sequencing we are now able to isolate and identify microorganisms which exist underneath the ocean, rivers and undergrounds. These studies (particularly using 16S rDNA) have enabled us to identify some anaerobic microbes, such as Clostridium, which degrades PCBs, which have not been identified before, even though scientists have tried for last 10-12 years. We are also using a white rot fungus Phanerochaete chrysosporium in biotransformation studies and in the search for 2,4-DNT degrading gene(s), which is one of the toxic metabolites of TNT. We have demonstrated that Rhizobium sp. can also bioremediate contaminated soil with petroleum products like toluene. Several undergraduate, graduate and post-doctoral students are currently working on various funded projects in our well equipped laboratories. We have published five books and more than 165 articles in various journals. Dr. Dutta and his several students have received national recognitions and monetary awards from various sources. TEACHING: Molecular Genetics (Both undergraduate and graduate levels). The course deals with molecular genome analysis, gene isolation using DNA:DNA and DNA:RNA re-association kinetics, molecular evolution, microbial ecology using 16S rDNA-ARDRA (Amplified Ribosomal DNA Restriction Analysis) techniques, genes for bioremediation, and DNA-Diagnostic tools (RFLP, RT-PCR, DNA-Chips and Microarray). Other courses taught are general Biology and general Microbiology. AWARDS: Current recipient of Agilent Instrument Award for LC-EIS-TOF-MS (PI. Dr. F. Ayorinde & Co-PI Dr. S. K. Dutta). First Prize for outstanding faculty research by ASB (American Society of South-Eastern Biologists) 2004; Faculty Author Awards 1996-2005; Nominated for Life time Mentoring Award by AAAs 2002. Outstanding Faculty Award by Howard University Students (1994); Distinguished Teacher/Scholar Award (1994), Howard University Cluster; HALL OF FAME for contribution in Molecular Genetics; NIH recognition as Outstanding Minority Student Mentor in Research (1998); Outstanding Research Award, College of Liberal Arts, 1983; Great Teacher Award by MERCK Pharmaceutical Company; WHO’S WHO of USA and the World; WHO’S WHO in Frontier Sciences; Recipient of approx. 5 million dollars research grants as PI and several million more dollars Institutional Training grants. STUDENT AWARDS AND MENTORING: First Prize winner in the Graduate Research Symposium 2005 (Supriyo De) for PhD work, Howard University, Washington DC; US/India research award (Supriyo De) 2005-2006. Howard University, Washington DC; First Prize winner in the Graduate Research Symposium 2002 (Supriyo De) for MS work. Howard University, Washington DC. Several other graduate students: Michelle Jackson, Christine Barrow, LaShaun Drew, Gail Hollowell, Gregory Davenport, Christie Maggee and several other graduate students received several awards from Graduate school and at national and international levels. Mentors for following numbers of students; Undergraduate Research Students including Minority Alliance Program- 45; Numbers of graduate (M.S. & Ph.D.) research students-90; Numbers of post-doctoral research students-25; Judges in local elementary, middle, and high school science fairs-4; Total numbers of research students trained in the areas of molecular genetics during 38 years tenure at Howard University- 162. Most of his prior students are currently holding prized positions in U.S Government Offices, in Universities and in Industries. PUBLICATIONS: Publications Beginning January 2006 (selected out of 165+ peer reviewed journal papers) De S, Perkins M, Dutta SK., Nitrate Reductase Gene Involvement in Hexachlorobiphenyl Dechlorination by Phanerochaete chrysosporium. J. Hazardous Materials 2006, (In press; doi:10.1016/j.jhazmat.2005.11.073). Chen Y-Q, Adam A, Toure O, and Dutta SK, Molecular evidence of genetic modification of Sinorhizobium meliloti : enhanced PCB bioremediation, J. Indus. Microbiol. Biotechnol., 32: 77-85, 2005. Perkins MW, De S, Frederick L, Dutta S K ,Ligninolytic Mineralization of Trinitrotoluene by Several White Rot Basidiomycetes. J. of Bioremediation, 9: 77-85, 2005. De S, Pramanik SK, Williams AL, Dutta S K, Toxicity of Polychlorobiphenyls and its Bioremediation. Int. J. of Human Genetics, 4: 281-290, 2004. Banerjee H., Verma M., Dutta S.K.; 2-Am,4,6-DNT Causes Genotoxicity of P53 Gene in NG108 Neuroblastoma Cell Lines, Int. J. Hum. Genet., 4: 227-229, 2004. Dutta, S.K., Adam A., Toure O., Williams A.L. and Chen Y.Q. Indigenous mixed soil bacteria in presence of compatible plants are more efficient in PCB degradation. F. Environ. Bull, 12: 314-319, 2003. Toure, O., Chen Y.Q., and Dutta S.K. Sinorhizobium meliloti electrotransporant containing ortho-dechlorination gene shows enhanced PCB-dechlorination. F. Environ. Bull, 12:, 320-322, 2003. Dutta, S.K., Hollowell, G.P., Fawzy, M.H., and Kuykendall, L.D. Genetically modified Sinorhizobium meliloti significantly enhances 2,4-DNT contaminated soil bioremediation. Soil Biol. and Biochem. 35: 667-675, 2003. Drew L and Dutta S.K. DNA diagnostic tools for β-hemoglobinopathies. Proceeding 10 -12, 2001. Dutta, S.K. and Toure, O. Accelerated Depletion of 2’, 3, 4 PCB congener using combinations of alfalfa plant and bacterial species. Proc. Hazardous Waste Research. pp. 1-4, 2000. Hou Li-Hua and Dutta S.K.. Phylogenetic characterization of several para-and meta-PCB dechlorinating Clostridium species: 16S rDNA sequence analyses. Letters in Applied Microbiology, 30: 238 -243, 2000. Jackson, M.M., Hou, L.-H., Banerjee, H.N., Sridhar, R., and Dutta, S.K. Disappearance of 2,4-dinitrotoluene and 2-amino, 4, 6-dinitrotoluene by Phanerocheate chrysosporium under non-lignolytic conditions. Bull. Environ. Contam. Toxicol. 62:390-396, 1999. Hollowell G.P., Kukendall L.D., Gillette W.K., Hashrem F.M., Hou L.H., Tatem H.E., and Dutta S.K. Genetic transfer and expression of plasmid RP4:TOL in Sinorhizobium meliloti, Bradyrhizobium japonicum and B. elkanii. Soil Biology and Biochemistry 31: 1811-1819, 1999. Dutta, S.K., Jackson, M.M., Hou, L.-H., Powell, D., and Tatem, H. Non-ligninolytic TNT mineralization in contaminated soil by Phanerochaete chrysosporium. Bioremediation J. 2(2): 97-103, 1998. Dutta, S.K., Nazar, A.S.M.I. and Verma, M.: Enolase activity in chicken embryo primary retina cells is not affected by exposure to a 60-Hz electric field. Cancer Biochemistry Biophysics 16: 45-52, 1998. LaMontagne, M.G., Davenport, G.J., Hou, L-H., and Dutta, S.K. Identification and analysis of PCB dechlorinating anaerobic enrichments by amplification: Accuracy of community structure based on restriction analysis and partial sequencing of 16S rRNA genes. J. Appl. Microbiol. 84(6): 1156-1162, 1998. Dutta S.K., Hou L.H. , Jackson M.M. and Tatem H.E. In situ disappearance of TNT from contaminated soils using microbes and co-metabolites. Proc. WERC/HSRC Cont. on the Environment 111-112, 1997. Kuykendall, L.D., Gillette, W.K., Hollowell, G.P., Hou, L.-H., Tatem, H.E., and Dutta, S.K.: Transfer Plasmid RP4::TOL to Legume Microsymbionts for Soil/Rhizosphere Bioremediation. In In Situ and On-Site Bioremediation, Vol. 4 (4): Battelle Press, Columbus, OH, pp65-70, 1997. Nazar, A.S.M.I., Paul, A. and Dutta, S.K.: Frequency-dependent alteration of enolase activity by ELF fields. Bioelectrochemistry and Bioenergetics 39: 259-262, 1996. Verma, M. and Dutta, S.K.: Transcription initiation and termination sites of ribosomal RNA gene clusters of Neurospora crassa. The Nucleus 39(1,2): 35-42, 1996. Dutta S.K. and Verma, M.: New directions in genetic-therapy research. Ind.J.Hum.Genet. 2: 13-18, 1996. Verma, M and Dutta S.K.: Transcription initiation and termination sites of ribosomal RNA gene clusters of Neurospora crassa. The Nucleus 39 (1,2): 35-42, 1996. Verma, M. and Dutta, S.K.: DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899, 1994. Dutta, S.K., Verma, M., and Blackman, C.F.: ELF frequency-dependent alterations in enolase activity in Esherichia coli caused by exposure to electric and magnetic fields. Bioelectromagnetics 15: 377-383, 1994. Nazar, A.S.M.I. and Dutta, S.K.: Effect of ELF magnetic fields on enolase activity. Electro and Magnetobiol. 13: 175-181, 1994. Kuykendall, L.D., Gaur, Y.D, and Dutta, S.K.: Genetic diversity among Rhizobium strains from Cicer arietinum L. Letters in Applied Microbiol, 17: 259-263, 1993. Banerjee, D., Schnieders, B., Bertino, J.R. and Dutta, S.K. Detection of a novel double mutation in a ?-thalassemia patient by RNA single-stranded conformation polymorphisms and direct sequencing. Blood 82: 3786-3792, 1993. Banerjee D, Talukder G, Tiwari R, Bhattacharya DK, Pakrashi A, Sharma A, Banerjee A, De M, Winter WP, Dutta SK: Molecular diagnosis of several β-Thalassemic mutations in patients from Calcutta, West Bengal populations using DNA probes and PCR. Nucleus, 35: 106-110, 1992. Dutta, S.K. & Verma, M.: Primary structure of the non-transcribed spacer region and flanking sequences of the ribosomal DNA of Neurospora crassa and comparison with other organisms. Biochem. Biophys. Res. Comm. 170: 187-193, 1990. Dutta, S.K., Bhattacharyya, Nandan, Parui, R. & Verma, Mukesh: Expression of DNA sequences containing neuron specific enolase gene in Escherichia coli. Biochem. Biophy. Res. Comm.173: 231-239, 1990. Dutta, S.K. & Verma, M. Isolation and characterization of non-neuronal enolase (NSE) from Neurospora crassa and comparison with neuron specific enolase isolated from neuroblastoma cell line NG108. Biochem. Intl. 15: 223-234, 1987. Dutta, S.K., Mukhopadhyay, D.K. & Bhattacharya, J. RNase sensitive DNA polymerase activity in cell fractions and mutants of N.crassa. Biochem. Genet. 18: 743-753, 1980. Dutta, S.K., Beljanski, M. & Bourgarel, P.: Endogenous RNA bound RNA- dependent DNA polymerase activity in N. crassa. Exptl. Mycol. 1: 173-180, 1976. Chattopadhyay, S.K., Kohne, D.E. & Dutta, S.K. Ribosomal RNA genes of Neurospora: Isolation and characterization. Proc. Natl. Acad. Sci. USA 69: 3256-3259, 1972. Garber, E.D. & Dutta, S.K. Evidence of mixed cytoplasm in heterocaryons of C.lagenarium. Science 135: 665, 1962. BOOKS Dutta, S.K. and Sloger, C. (Eds.). Nitrogen Fixation Associated with Rice Production. Oxford & IBH Publishing Co., New Delhi, India (1991). ISBN # 81-204-0560-9. Dutta, S.K. and Winter, W.P. (eds.). DNA Systematics: Human and Higher Primates, Vol. III, CRC Press, Florida (1990). ISBN # 0-8493-5822-1. Dutta, S.K. and Millis, R.M. (eds.). Biological Effects of Electropollution: Brain Tumors and Experimental Models. Information Ventures, Inc., Philadelphia, PA (1986). ISBN # 0-9617314-1-9. Dutta, S.K. (ed.). DNA Systematics: General, Vol., CRC Press, Florida (1986). ISBN # 0-8493-5820-5. Dutta, S.K. (ed.). DNA Systematics: Plants, Vol. II., CRC press, Florida (1986). ISBN # 0-8493-5821-3.