DEPARTMENT OF BIOLOGY FACULTY PROFILE
RESEARCH MICHAEL WHEELER LIPSCOMB PUBLICATIONS

Contact Information
Michael W. Lipscomb, PhD
Associate Professor - Department of Biology
E-mail: michael.lipscomb@howard.edu
Office Number: 202-677-3097
Laboratory Location: EE Just Hall Room G32
Laboratory Telephone: 202-806-7939

Link to Google Scholar: Michael Lipscomb

Education
B.S., University of California, Los Angeles (2003)
M.S., Cal State University, Los Angeles (2005)
Ph.D., University of Pittsburgh, School of Medicine (2009)
Postdoctoral Fellow, University of Pennsylvania (2009-2011)

Courses
Cell Biology (BIOL310)
Cancer Biology (BIOL420)
Immunology (BIOL422)

Grants and Funding Awards
National Institutes of Health SC1GM127207 2018-2023 [PI]
National Institutes of Health SC2GM103741 2015-2019 [PI]
Fulbright Scholar-Brazilian Mobility 2015-2017 [PI]
National Science Foundation DBI-1428768 2014-2016 [PI]
Department of Defense W911NF-14-1-0123 2013-2015 [PI]

Research Interests
Immunoregulatory network of antigen presentation in dendritic cells
Autoimmunity and immunopathologies associated with Type 1 diabetes
Dendritic cell-based immunotherapies and novel insulin restorative therapies

Laboratory Personnel
Diana Elizondo, PhD - Research Scientist - FLARE Fellow 2018 - ResearchGate Profile
Nailah Brandy, PhD - Research Associate - ResearchGate Profile
Temesgen Andargie, MS - Research Technician - ResearchGate Profile
Ricardo da Silva, PhD - Postdoctoral Fellow - ResearchGate Profile
Leslie Goode, LATG - Animal Technician

The Endocrine Society - Published on Mar 19, 2018 - This press conference explores efforts to reverse diabetes with implanted beta cells, the development of a wearable patch to detect dangerous episodes of low blood sugar called hypoglycemia, and the effectiveness of a hybrid closed-loop system for managing type 1 diabetes
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  Research Detailed

Dendritic cell Biology and Antigen Presentation to T cells

Using transcriptomics and bioinformatics, the laboratory aims to identify and characterize novel genes expressed in dendritic cell (DC) subsets that govern both (1) differentiation from hematopoietic stem cells and (2) antigen presentation capacity to T cells. Using a combination of transcriptomic profiling, RNA interference, which include both siRNA- and CRISPR/Cas9-mediated silencing, and transgenic mouse models, mechanisms that govern the immunoregulatory pathway are delineated through biochemical, molecular, and cellular experimental approaches. The laboratory has uncovered novel roles of allograft inflammatory factor -1 (AIF1), A disintegrin and metalloprotease 23 (ADAM23), and drebrin 1 (DBN1) in mediating differentiation and/or antigen-presentation capacities of the cross-presenting subsets of CD11c+ dendritic cells. Continued studies aim to further uncover these proteins' functional role in the larger immnoregulatory pathways that dictate immunity vs. tolerance. Additional genes that show high correlative index in expression profile are also being pursued in the laboratory, which has now led into studies of macrophage differentiation and inflammatory roles in viral and parasitic infections. These studies are in part through collaborations with research teams at the Federal University of Segipe in Brazil.

Autoimmunity, Type 1 Diabetes, Immunotherapies and Insulin Restorative Therapies

Current therapies of type 1 diabetes are limited to non-specific global immune suppressive drugs, allogeneic transplantations, and administration of exogenous insulin. These approaches can cause a myriad of opportunistic infections and deregulation of host cellular processes (i.e. impaired endocrine signaling and disrupted hepatic functioning). The laboraotry's investigations aim to develop innovative immunotherapeutic approaches to inhibiting autoimmune destruction of beta-cells. The treatment application(s) employs sophisticated dendritic cell-based vaccine and adoptive T cell therapy strategies to induce antigen-specific tolerance to autoreactive T cells, as well as be used to suppress tissue-specific pro-inflammatory responses. In conjugation, methods of restorative insulin therapy using novel synthetic polymers for adoptive implantation therapies are also employed to restore physiologically-regulated/controlled insulin levels and alleviating hyperglycemia in diabetic individuals.





Selected Publications

  Elizondo DM, Andargie TE, Kacsinta AD, Silva R, Moura T, Lipscomb MW. Allograft Inflammatory Factor-1 governs hematopoietic stem cell differentiation into MHC class II+ CD11c+ dendritic cells. Frontiers in Immunology. under review
  Elizondo DM, Andargie TE, Kacsinta AD, Silva R, Moura T, Lipscomb MW. IL-10 producing CD122+ PD-1+ CD8+ T regulatory cells are expanded by dendritic cells silenced for Allograft Inflammatory Factor-1. Journal of Leukocyte Biology. 2018
  Elizondo DM, Andargie TE, Boddie TA, Haddock NL, Lipscomb MW. Drebrin 1 in dendritic cells regulates phagocytosis and membrane-bound receptor expression through recycling for efficient antigen presentation. Immunology. 2018.
  Lucas S Magalhães, Lays GS Bomfim, Sthefanne G Mota, Geydson S Cruz, Cristiane B Corrêa, Diego M Tanajura, Michael W Lipscomb, Valéria M Borges, Amélia R de Jesus, Roque P de Almeida, Tatiana R de Moura. Increased thiol levels in Leishmania infantum resistant to antimony isolated from visceral leishmaniasis refractory to treatment from Brazil. Meorias Do Instituto Oswaldo Cruz. 2018
  Elizondo DM, Andargie TE, Yang D, Kacsinta AD, Lipscomb MW. Inhibition of Allograft Inflammatory Factor-1 in dendritic cells restrains CD4+ T cell effector responses and induces CD25+Foxp3+ T regulatory subsets. Frontiers in Immunology. 2017
  Elizondo DM, Andargie TE, Kubar DM, Gugssa A, Lipscomb MW. CD40-CD40L crosstalk drives fascin expression in dendritic cells for efficient antigen presentation to CD4+ T cells. International Immunology. 2017
  Elizondo DM, Andargie TE, Marshall KM, Zariwala AM, Lee CM, Lipscomb MW. siRNA Immunological Fishing Training (SIFT) experience as a novel research education tool for students studying immunology. Journal of Microbiology and Biology Education (JMBE). 2017
  Ricardo da Silva, Marcio Santos, Priscila Almeida, Thayse Barros, Lucas Magalhães, Rodrigo Cazzaniga, Patrícia Souza, Valeria Borges, Nivea Luz, Jaqueline França-Costa, Djalma Lima-Junior, Michael Lipscomb, Malcom Duthie, Steven Reed, Roque Almeida, and Amelia de Jesus. Circulating CD163 levels are indicative of infection and disease status in leprosy and Leishmaniasis. PLoS Neglected Tropical Diseases. PLoS Negl Trop Dis. 2017 Mar 29;11(3):e0005486. 2017
  Lays Gisele Santos Bomfim, Lucas Sousa Magalhães, Marcelo Augusto Anchieta Santos Filho, Nalu Teixeira de Aguiar Peres, Cristiane Bani Corrêa, Diego Moura Tanajura, Angela Maria Silva, Michael Wheeler Lipscomb, Valeria Matos Borges, Amélia Ribeiro Jesus, Roque Pacheco De Almeida, Tatiana Rodrigues De Moura. Leishmania infantum induces the release of sTREM-1 in visceral leishmaniasis. Frontiers in Microbiology. 2017
  Roseane Nunes de Santana Campos; Márcio Bezerra Santos; Gabriel Isaias Lee Tunon; Luana Celina Seraphim Cunha; Lucas Sousa Magalhães; Michael Lipscomb; Juliana Lima Silva Moraes; Danielle Melo S. Ramalho; Sanmy Silveira Lima; José Antônio Pacheco de Almeida; Amélia Ribeiro de Jesus; Roque Pacheco de Almeida. Epidemiological aspects and spatial analysis of human and canine visceral leishmaniasis in an endemic area in north-eastern Brazil. Geospatial Health. 2017
  Elizondo DM, Andargie TE, Marshall KM, Zariwala AM, Lipscomb MW. Dendritic cell expression of ADAM23 governs T cell proliferation and cytokine production through the a(v)ß(3) integrin receptor. J Leukoc Biol. 2016 Nov;100(5):855-864
  Mwendwa DT, Ali MK, Sims RC, Cole AP, Lipscomb MW, Levy SA, Callender CO, Campbell AL. Dispositional depression and hostility are associated with inflammatory markers of cardiovascular disease in African Americans. Brain Behav Immun. 2013 Feb;28:72-82
  Yamakita Y, Matsumura F, Lipscomb MW, Chou PC, Werlen G, Burkhardt JK, Yamashiro S. Fascin1 promotes cell migration of mature dendritic cells. 2011 Mar 1;186(5):2850-9. Epub 2011 Jan 24.
  Lipscomb MW, Taylor JL, Goldbach CJ, Watkins SC, Wesa AK, Storkus WJ. DC expressing transgene Foxp3 are regulatory APC. Eur J Immunol. 2010 Feb;40(2):480-93
  Lipscomb MW, Chen L, Taylor JL, Goldbach C, Watkins SC, Kalinski P, Butterfield LH, Wesa AK, Storkus WJ. Ectopic T-bet expression licenses dendritic cells for IL-12-independent priming of type 1 T cells in vitro. J Immunol. 2009 Dec 1;183(11):7250-8. Epub 2009 Nov 13.


Equipment in the Lipscomb Laboratory

- 1 BD FACSVerse flow cytometric analyzer (405, 488, and 640nm laser excitation; 10-parameter)
- 1 BD FACSJazz flow cytometric sorter (488 and 640nm laser excitation; 7-parameter)
- 1 BD Accuri C6 flow cytometric analyzer (488 and 640nm laser excitation; 6-parameter)
- 1 Luminex Magpix multiplex microplate reader system with xMAP technology
- 1 Biotek HT multimode fluorescence, luminescence, and absorbance microplate reader
- 1 Licor Odyssey infra-red two laser-scanning nucleic acid and protein imaging system
- 1 Cryostar NX70 cryogenic sectioner with full automation
- 1 Olympus FSX100 (3-Channel; 350, 488, 561nm) XYZ-motorized fluorescent microscope
- 1 Olympus SZ61 40X Stereomicroscope with attached Digital Camera (488 and 561nm)
- 2 EVOS FL manual inverted microscopes (DAPI, GFP, Cy5.5, Cy7 Texas Red; interchangeable cubes)
- 1 Countess II automated fluorescent cell counter (DAPI, GFP, RFP, Texas Red, Cy5.5, and Cy7)
- 1 Thermo Fisher QuantStudio 5 Real Time qPCR machine
- 1 Thermo Fisher Veriti Gradient thermocycler
- 1 BioRad T100 thermocycler
- 1 NanoDrop Lite connected to 1 laptop
- 1 ECM 830 square wave electroporation unit
- 1 Miltenyi GentleMACS Dissasociator
- 1 Thermo Fisher Scientific 5 ft. Class II Type A2 Biological Safety Cabinet
- 2 Thermo Fisher Scientific CO2 incubators; 1 direct heat injection and 1 water-jacketed
- 1 Quincy 140 series standard incubator dedicated for bacterial cultures
- 1 Allegra X-12R refrigerated benchtop centrifuge with microplate/tube interchange assembly
- 1 Allegra X-30 benchtop centrifuge with microplate/tube interchange assembly
- 2 Microcentrifuge-16 centrifuges; 1 refrigerated and 1 room temperature
- 1 MaxQ Orbital shaker incubation unit; fits 250 mL and 50 mL flask sizes
- 2 Lonza FlashGels plus image capturing device (for nucleic acid analysis, capture and extraction)
- 1 UV/Visi Blue Protein and Gel Blot Reader
- 1 Benchmark BioClave™ Research Benchtop Autoclave
- 6 power supplies for (nucleic acid and protein) electrophoresis apparatuses
- 1 Thermo Scientific Locator Jr Liquid Nitrogen Dewar
- 1 minus 86 C ultralow freezer with digital ambient recorder
- 1 minus 20 C freezer with digital ambient recorder
- 1 4C dual refrigerator with digital ambient recorder
- 1 4C under counter refrigerator dedicated to antibodies and bioreagents; wireless monitoring system